RS & RS Scientific Service Platform Overview

Our protein isolation service is tailored to extract high-quality proteins from various biological samples. Using optimized lysis buffers, protease inhibitors, and precise centrifugation steps, we ensure maximal yield and integrity of the target proteins.

How We Do It:

• Sample Preparation: Careful homogenization and lysis of tissues or cells.

• Buffer Optimization: Customized extraction buffers to suit specific proteins.

• Purification & Quality Check: Sequential purification steps followed by quantification and quality assessment using spectrophotometry and SDS-PAGE.

The Western blot service offers qualitative and semi-quantitative protein detection and analysis. This method provides critical insights into protein expression, modifications, and integrity.

How We Do It:

• Electrophoresis & Transfer: Separation of proteins via SDS-PAGE followed by efficient transfer onto membranes.

• Immunodetection: Specific primary antibodies and HRP-conjugated secondary antibodies are used to detect target proteins.

• Visualization & Analysis: Enhanced chemiluminescent detection coupled with digital imaging for accurate quantification and analysis.

We offer a broad range of cell culture assays to assess cell viability, proliferation, migration, invasion, and other critical cellular functions. Our expertise in cell biology and assay development guarantees reliable and reproducible results.

MTT Assay
Purpose: Assess cell viability and metabolic activity.
Method: Cells are treated with MTT reagent, which is metabolically reduced by viable cells into a formazan product. The intensity of the color is proportional to the number of viable cells.

Proliferation Assay
Purpose: Measure the rate of cell division over time.
Method: Utilizing colorimetric or fluorescence-based markers, we quantify DNA synthesis or cell number increases to determine proliferation rates.

Migration and Wound Healing Assays
Purpose: Evaluate the ability of cells to migrate and close a wound gap.
Method: A scratch is introduced into a cell monolayer, and the rate of gap closure is monitored over time, providing insights into cell motility and migration dynamics.

Invasion Assay
Purpose: Determine the invasive potential of cells through extracellular matrix components.
Method: Cells are seeded in invasion chambers coated with Matrigel, simulating the basement membrane, to assess their ability to degrade and traverse the barrier.

Colony Forming Assay
Purpose: Assess the clonogenic ability of cells to form colonies, indicative of long-term proliferative potential.
Method: Single cells are seeded at low density, and colony formation is monitored over days to weeks, with subsequent staining and quantification.

Spheroid Assay
Purpose: Model three-dimensional cell culture to better mimic in vivo tumor conditions.
Method: Cells are cultured in non-adherent conditions to form spheroids, which are then used to assess responses to drugs or genetic modifications in a 3D context.

Our immunofluorescence service offers high-resolution localization and visualization of target proteins within cells and tissues. By combining specific antibody labeling with advanced imaging techniques, we enable detailed analysis of protein distribution, cellular architecture, and dynamic biological processes.

How We Do It:

Sample Preparation: Cells or tissue sections are fixed and permeabilized to preserve cellular structures and antigenicity.

Antibody Labeling: High-affinity primary antibodies specific to the target proteins are applied, followed by fluorophore-conjugated secondary antibodies for precise detection.

Imaging: Advanced fluorescence microscopy is used to capture detailed images, and image analysis software is employed to quantify and analyze protein localization and expression patterns.

Quality Control: Each assay is validated with appropriate controls to ensure specificity and reproducibility.

Our cloning service is designed to generate recombinant DNA constructs tailored for gene expression, protein production, or functional analysis. We utilize state-of-the-art molecular cloning techniques to deliver precise and reliable constructs for downstream applications.

How We Do It:

• Vector & Insert Design: Customized design of vectors and target gene inserts using advanced bioinformatics tools.

• Molecular Assembly: Employing a variety of cloning techniques—including restriction enzyme-based cloning, Gibson Assembly, or ligation-independent cloning—to ensure optimal construct assembly.

• Transformation & Screening: Introduction of recombinant plasmids into competent bacterial cells, followed by rigorous colony screening and validation (via PCR, restriction digest, and sequencing) to confirm the integrity of the clone.

Our ELISA services enable the quantitative detection of proteins, antibodies, and other analytes. We apply highly specific antibody-antigen interactions to deliver sensitive and specific assay results.

How We Do It:

• Plate Coating & Blocking: Optimized coating of microplates with capture antibodies and effective blocking to minimize non-specific binding.

• Detection: Utilization of enzyme-conjugated secondary antibodies and chromogenic substrates to generate quantifiable signals.

• Data Analysis: Standard curve generation and rigorous data validation to ensure accuracy and reproducibility.

Our PCR and qPCR services enable amplification and quantification of nucleic acids with high sensitivity and specificity. These techniques are critical for gene expression analysis, pathogen detection, and genetic profiling.

How We Do It:

• PCR Setup: Meticulous design of primers, optimization of reaction conditions, and stringent quality controls.

• Thermal Cycling: Utilization of state-of-the-art thermal cyclers to ensure efficient and specific DNA amplification.

• qPCR Analysis: For qPCR, fluorescence-based detection and precise quantification are achieved with robust internal controls and standard curves, providing real-time quantification of target sequences.

Our viral packaging services provide a streamlined approach for generating viral vectors used in gene delivery applications. We offer packaging for various viral systems to support research in gene therapy, functional genomics, and transduction-based assays.

How We Do It:

• Vector Design & Preparation: Customized vector design according to experimental needs, including lentivirus, adenovirus, or adeno-associated virus (AAV) systems.

• Packaging & Production: Transfection of packaging cell lines under optimized conditions to produce high-titer viral particles.

• Quality Control: Viral titration, purity assessment, and functionality testing ensure the reliability of produced viral vectors.

Our immunoprecipitation service is designed to isolate and concentrate a specific protein or protein complex from cell or tissue lysates. This technique is crucial for studying protein-protein interactions, post-translational modifications, and signaling pathways.

How We Do It:

Antibody Selection: Use of high-affinity, validated antibodies specific to the target protein.

Binding & Capture: Incubation of lysates with antibody-bound beads to selectively capture the target proteins.

Washing & Elution: Rigorous washing steps to remove non-specifically bound proteins, followed by elution of the immunoprecipitated proteins for downstream analyses such as Western blot or mass spectrometry.